These standards can be detected by fluorescence when excited at appropriate wavelengths. Thermo Scientific PageRuler Prestained Protein Ladder is a mixture of 10 blue-, orange-, and green-stained proteins (10 to 180 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and western blotting. Figure 1: Molecular weight of the marker bands when using different buffer. mPAGE ® Western Protein Standards consist of seven recombinant proteins at 20 kDa, 30 kDa, 40 kDa, 50 kDa, 60 kDa, 80 kDa and 120 kDa with an IgG binding site that binds to most antibodies. Precision Plus Protein Kaleidoscope Standards are a mixture of ten multicolored recombinant proteins (10–250 kD). The BlueStar Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon or nitrocellulose) and for approximating the size of proteins. ![]() Double the loading volume for thicker or larger gel.Place this handy selection guide magnet on your refrigerator or lab bench to eliminate the guesswork and make protein standard selection simple. The BLUeye Prestained Protein Ladder is a three-color protein standard with 12 prestained proteins covering a wide range molecular weights from 10 to 245. Use Precision Plus Protein WesternC Standards as a combination standard for western blotting and fluorescence detection in SDS-PAGE.1.5 – 2.5µl per loading for general Western transferring.Note for image: The apparent molecular weight of each protein (kDa) has. Compare all Protein standards and molecular weight ladders. Protein ladders for IEF, native PAGE and specialty applications. Protein ladders for western blotting and fluorescence western blotting. After transferring the protein bands onto the blot paper, stain the paper with Ponceau-S. Tested in polyacrylamide gel electrophoresis and Western Blotting. On this page: Prestained broad and high molecular weight protein ladders. Load ladder into SDS-PAGE gel or Western blot using these volumes (based on gel thickness of 0.75 – 1.0mm). If you want to do with unstained ladder yet, I recommend to do all steps of western blot just routinely do.Mix solution gently to ensure that it is homogeneous. The Pre-Stained Protein Ladder is designed for monitoring protein separation during SDSpolyacrylamide gel electrophoresis, verification of Western transfer.Thaw ladder at room temperature to dissolve precipitated solids.Used in SDS-PAGE and Western blots for approximation of protein sizes, and also to locate a protein of interest for excision from an unstained preparative gel. Whether it is for protein electrophoresis or western blot, our pre-stained protein markers help you quickly determine the molecular weight of the target. Chameleon 700 or Chameleon 800 protein ladders can be used for one-channel analysis. This protein marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on. Western blotting protein standards can be used for both fluorescent visualization and colormetric or chemiluminescent immunodetection on western blots. ![]() No further dilution, addition of a reducing agent or heating is required.Ģ0mM Tris-H3PO4 (pH 7.5 25☌) 15% (v/v) Glycerol 2% (w/v) SDS 10mM DTT 3.6mM Urea Ensure good size coverage with Chameleon ladders ranging from 8 kDa to 260 kDa Easily visualize gel migration and protein size Orient your gel or membrane quickly Chameleon Pre-stained Protein Ladders are optimized for use with Bis-Tris and Tris-Glycine gels. An orange reference band of ~75kDa and a green reference band of ~25kDa provides easy monitoring of protein separation. Prestained Protein Ladder contains 13 prestained recombinant proteins that cover a wide range of molecular weights from 3.5kDa – 245kDa.
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